Tissue culture is defined as growing or culturing of desired cells, tissues, or organs on a designed sterile synthetic medium under controlled conditions of temperature, light, and humidity
Rapid Multiplication- Quantity with Quality
Disease Free Plants
Production of Transgenic plants/Genetic Engineering
Production of secondary Metabolites
Conservation of Species
Somoclonal variation
Plant stem cells
Mass Multiplication of Banana
Suckers
Field
Uniformity
Not True to type
Disease
Bunch size
Harvesting
Suckers
Field
Uniformity
True to type
Disease free
Uniform Bunch size
Harvesting- 3-4 cuttings
Selection of Mother Plants
Identification of Plot
Marking of Selected plants
Sword sucker/Water Suckers
No of Finger’s
Length of Finger’s
Distance between hand’s
No of hand’s per bunch
Disinfection of Sucker’s
Stages in Banana Tissue Culture- In Vitro
Stages in Banana Tissue Culture- Hardening
Primary hardening
Secondary hardening
Mass multiplication of Phalaenopsis Species
Pods
Germination
Seedlings
Protocorm Like Bodies
True to Type Plants
High Mortality during hardening
Quality to meet International Standards
Economical & Rapid Scale up
Mass multiplication of Phalaenopsis Species
Direct Regeneration
Less than 1% offtypes
Less than 5% Mortality during hardening
Quality to meet International Standards
Economical & Rapid Scale up ( Single node= 2k seedlings)
Ex-Agar
Seedling
Matured
Blooming
Commercial Infrastructure
(Mass Multiplication)
Non-Sterile Area
Washing
Decontamination
Plant washing
Plant grading
Semi-Sterile Area
Media Preparation
Media filling
Media Sterilization
Sterile Area
Sterilized Media Storage
Inoculation Room
Growth Rooms
Primary Hardening
Green house/ Polyhouse
Secondary Hardening
Polyhouse / Shade Net
Glowing Phalaenopsis Orchids:
The next generation of flowering plants