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Plant Tissue Culture

Tissue culture is defined as growing or culturing of desired cells, tissues, or organs on a designed sterile synthetic medium under controlled conditions of temperature, light, and humidity 

  • Rapid Multiplication- Quantity with Quality
  • Disease Free Plants
  • Production of Transgenic plants/Genetic Engineering
  • Production of secondary Metabolites
  • Conservation of Species
  • Somoclonal variation
  • Plant stem cells

Mass Multiplication of Banana

Suckers

Field

  • Uniformity
  • Not True to type
  • Disease
  • Bunch size
  • Harvesting

Suckers

Field

  • Uniformity
  • True to type
  • Disease free
  • Uniform Bunch size
  • Harvesting- 3-4 cuttings

Selection of Mother Plants

Identification of Plot

Marking of Selected plants

Sword sucker/Water Suckers

  • No of Finger’s
  • Length of Finger’s
  • Distance between hand’s
  • No of hand’s per bunch

Disinfection of Sucker’s


Stages in Banana Tissue Culture- In Vitro


Stages in Banana Tissue Culture- Hardening

Primary hardening

Secondary hardening


Mass multiplication of Phalaenopsis Species

Pods

Germination

Seedlings

Protocorm Like Bodies

  • True to Type Plants
  • High Mortality during  hardening
  • Quality to meet International Standards
  • Economical  & Rapid Scale up

Mass multiplication of Phalaenopsis Species

Direct Regeneration

  • Less than 1% offtypes
  • Less than 5% Mortality during hardening
  • Quality to meet International Standards
  • Economical &  Rapid Scale up ( Single node= 2k seedlings)

Ex-Agar

Seedling

Matured

Blooming


Commercial Infrastructure (Mass Multiplication)

Non-Sterile Area

  • Washing
  • Decontamination
  • Plant washing
  • Plant grading

Semi-Sterile Area

  • Media Preparation
  • Media filling
  • Media Sterilization

Sterile Area

  • Sterilized Media Storage
  • Inoculation Room
  • Growth Rooms

Primary Hardening

  • Green house/ Polyhouse

Secondary Hardening

  • Polyhouse / Shade Net

Glowing Phalaenopsis Orchids: The next generation of flowering plants

G9/William/Yellaki

Phalaenopsis

Cordyline

Syngoniums

Ficus